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Benzonase Nuclease derived from Serratia marcescens expressed in E.Coli

Shanghai Yaxin Biotechnology Co.,Ltd.

Benzonase Nuclease derived from Serratia marcescens expressed in E.Coli

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Brand Name : YAXINBIO

Model Number : RSN20

Certification : NQA ISO 9001:2015

Place of Origin : China, Shanghai

Payment Terms : T/T

MOQ : Negotiation

Price : Negotiation

Supply Ability : 1000kg/month

Catalog Number : RSN20

Cas : 9025-65-4

EC : 3.1.30.2

Storage Temperature : -20℃

Appearance : Liquid

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Super Nuclease derived from Serratia marcescens expressed in E.Coli

Super Nuclease

1.Description

Benzonase Nuclease, Synonyms:Benzonase Endonuclease,Super Nuclease,TurboNuclease,Universal Nuclease,a non-specific endonuclease derived from Serratia marcescens. Yaxin Benzonase Nuclease has a high activity and enzyme specificity, can cut between any nucleotides in the chain to completely digest the nucleic acid into 5'-monophosphate oligonucleotides with a length of 3-8 bases, which can degrade various forms of DNA and RNA (double-stranded, single-stranded, linear, circular, natural or denatured) under a wide range of conditions (6M Urea, 0.1M Guanidine HCl, 0.4%Triton X100, 0.1% SDS, 1mM EDTA, 1mM PMSF). This product has a wide range of uses, usually used in removing nucleic acids from biological products such as recombinant proteins, virus vaccines, and effectively reducing the viscosity of protein lysate samples such as cells, tissues, and microorganisms.

2.Main Features

Source Recombinant E. Coli
Appearance Liquid
Activity ≥ 250 U/μL

Electrophoresis

(Molecular weight)

27.9±2.8 kDa
Purity (Electrophoresis) ≥99%,≥90%
Buffer 20 mM Tris-HCL pH8.0, 2mM MgCl2, 2mM NaCl, 50% Glycerol
Endotoxin <0.25 EU/1000 U by LAL
Optimum pH 8.0-9.0
Optimum Temperature 37℃
Isoelectric Point 6.2
Cofactor Mg2+

One unit Nuclease is defined as the amount of enzyme that causes a ∆A260 of 1.0 (equivalent to the complete digestion of 37μg DNA) in 30min at 37°C, pH 8.0.

3.Stability of Storage

Storage stability: -20℃ within specification range for a period of at least 24 months .

Note: it is not recommended to store the product at -70℃ or below, since freezing the product will cause loss of acivity

4.Product Advantage

Animal origin free: No exogenous virus contamination,and any animal origin material is not used in the production process.

Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

High purity: Higher specific activity.Host protein residues is less than the limits of biological products.

Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

5.Usage

This product is used to reduce the viscosity of cell, tissue or bacterial lysate; remove nucleic acid in recombinant protein/recombinant virus; remove nucleic acid interference when determining recombinant virus titer by real-time quantitative PCR; prevent cell clumping; reduce the viscosity of E. coli lysate to improve Difficulty in centrifugation and filtration in the purification process; increase the refolding rate of inclusion body proteins; preparation of two-dimensional gel electrophoresis samples.


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